Peer-Reviewed Journal Details
Mandatory Fields
van Sluis, M,McStay, B
2017
June
Current Opinion In Cell Biology
Nucleolar reorganization in response to rDNA damage
Published
Altmetric: 2WOS: 8 ()
Optional Fields
DOUBLE-STRAND BREAKS POLYMERASE-I TRANSCRIPTION DNA-DAMAGE RIBOSOMAL-RNA CELL-CYCLE GENOMIC ARCHITECTURE ORGANIZER REGIONS REPAIR ATM CHROMATIN
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Nucleoli, sites of ribosome biogenesis, form around nucleolar organizer regions (NORs) comprising rDNA arrays, located on human acrocentric chromosome p-arms. NORs provide an opportunity to investigate the DNA double strand break (DSB) response at highly transcribed, repetitive, essential loci. Targeted introduction of DSBs into rDNA results in ATM-dependent inhibition of RNA-polymerase I transcription, coupled with movement of rDNA from the nucleolar interior to anchoring points at the periphery. Reorganization renders rDNA accessible to repair factors, normally excluded from nucleoli. Importantly, rDNA DSBs recruit the accurate homologous recombination (HR) repair machinery throughout the cell cycle, suggesting that HR can be templated in cis. We discuss recent findings regarding the biophysical properties of nucleoli and suggest a mechanism for stress-induced nucleolar reorganization.
10.1016/j.ceb.2017.03.004
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