Peer-Reviewed Journal Details
Mandatory Fields
Gerlach, JQ,Maguire, CM,Kruger, A,Joshi, L,Prina-Mello, A,Griffin, MD
2017
June
Nanomedicine
Urinary nanovesicles captured by lectins or antibodies demonstrate variations in size and surface glycosylation profile
Published
WOS: 5 ()
Optional Fields
capture exosome extracellular vesicle glycosylation isolation lectin nanotracking analysis precipitation urine NANOPARTICLE TRACKING ANALYSIS PROSTATE-CANCER CELLS EXOSOME-LIKE VESICLES EXTRACELLULAR VESICLES MICROVESICLES BIOMARKERS ULTRAFILTRATION IDENTIFICATION PURIFICATION ENRICHMENT
12
1217
1229
Aim: The use of carbohydrate-binding proteins (lectins) to isolate urinary extracellular vesicles (uEVs) was investigated and the captured subpopulations were characterized. Methods: Pooled uEVs from multiple healthy donors were exposed to lectin-conjugated or antibody-conjugated beads. Recovered uEVs were evaluated by protein estimation, transmission electron microscopy, nanoparticle tracking analysis and lectin microarray profiling. Results: uEVs isolated by lectin- and antibody-based affinity capture exhibited distinct variations in size and surface content. Transmission electron microscopy confirmed similar EV diameters to those established by nanoparticle tracking analysis, but total particle counts did not correlate closely with protein based quantification. Lectin microarray profiling demonstrated capture-dependent differences in surface glycosylation. Conclusion: Selective, carbohydrate-mediated EV isolation by lectin affinity approaches may prove immediately useful for research and find eventual use in clinical applications.
10.2217/nnm-2016-0016
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