Book Chapter Details
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Wells, K. E.,McMahon, J.,Foster, H.,Ferrer, A.,Wells, D. J.
2008 Unknown
Methods Mol Biol
Gene delivery to dystrophic muscle
Humana Press
Humana
Published
0
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Electroporation is a powerful method for gene delivery to dystrophic muscle in the mdx mouse model of Duchenne muscular dystrophy. Successful transfer of reporter and therapeutic plasmids and antisense oligonucleotides has been demonstrated. However, the efficiency falls with increasing plasmid size. Although it is unlikely that the electrotransfer approach will be useful clinically, it is an important experimental tool, particularly in testing potential immune responses to gene transfer in the absence of vector proteins.Electroporation is a powerful method for gene delivery to dystrophic muscle in the mdx mouse model of Duchenne muscular dystrophy. Successful transfer of reporter and therapeutic plasmids and antisense oligonucleotides has been demonstrated. However, the efficiency falls with increasing plasmid size. Although it is unlikely that the electrotransfer approach will be useful clinically, it is an important experimental tool, particularly in testing potential immune responses to gene transfer in the absence of vector proteins.
1064-3745 (Print)1064-37
http://www.ncbi.nlm.nih.gov/pubmed/18370219http://www.ncbi.nlm.nih.gov/pubmed/18370219
421
31
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