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Raimundo, SC,Pattathil, S,Eberhard, S,Hahn, MG,Popper, ZA
2017
March
Protoplasma
beta-1,3-Glucans are components of brown seaweed (Phaeophyceae) cell walls
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beta-1,3-Glucan Callose Immunolabeling LAMPmAb Brown seaweed Cell wall MONOCLONAL-ANTIBODIES LAMINARIA-DIGITATA ANILINE BLUE FUCUS-SERRATUS POLLEN TUBES SULFATED POLYSACCHARIDES ARABINOGALACTAN PROTEINS CARBOHYDRATE EPITOPES EXTRACELLULAR-MATRIX BIFURCARIA-BIFURCATA
254
997
1016
LAMP is a cell wall-directed monoclonal antibody (mAb) that recognizes a beta-(1,3)-glucan epitope. It has primarily been used in the immunolocalization of callose in vascular plant cell wall research. It was generated against a brown seaweed storage polysaccharide, laminarin, although it has not often been applied in algal research. We conducted in vitro (glycome profiling of cell wall extracts) and in situ (immunolabeling of sections) studies on the brown seaweeds Fucus vesiculosus (Fucales) and Laminaria digitata (Laminariales). Although glycome profiling did not give a positive signal with the LAMP mAb, this antibody clearly detected the presence of the beta-(1,3)-glucan in situ, showing that this epitope is a constituent of these brown algal cell walls. In F. vesiculosus, the beta-(1,3)-glucan epitope was present throughout the cell walls in all thallus parts; in L. digitata, the epitope was restricted to the sieve plates of the conductive elements. The sieve plate walls also stained with aniline blue, a fluorochrome used as a probe for callose. Enzymatic digestion with an endo-beta-(1,3)-glucanase removed the ability of the LAMP mAb to label the cell walls. Thus, beta-(1,3)-glucans are structural polysaccharides of F. vesiculosus cell walls and are integral components of the sieve plates in these brown seaweeds, reminiscent of plant callose.
10.1007/s00709-016-1007-6
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