Peer-Reviewed Journal Details
Mandatory Fields
Chora, S;McDonagh, B;Sheehan, D;Starita-Geribaldi, M;Romeo, M;Bebianno, MJ
2010
November
Chemosphere
Ubiquitination and carbonylation of proteins in the clam Ruditapes decussatus, exposed to nonylphenol using redox proteomics
Published
WOS: 10 ()
Optional Fields
MUSSEL MYTILUS-EDULIS ANTIOXIDANT ENZYME-ACTIVITIES OXIDATIVE-STRESS ENVIRONMENTAL-STRESS TAPES-PHILIPPINARUM LIPID-PEROXIDATION 4-NONYLPHENOL BIOACCUMULATION THIOLS ETHOXYLATES
81
1212
1217
Ubiquitination and carbonylation of proteins were investigated in the gill and digestive gland of Ruditapes decussatus exposed to NP (nonylphenol) (100 mu g L(-1)) using redox proteomics After 21 d of exposure clams were dissected and cytosolic proteins of both tissues separated by 2DE SDS-PAGE Protein expression profiles were tissue dependent and differently affected by NP exposure Ubiquitination and carbonylation were also tissue specific NP exposure induced significantly more ubiquitinated proteins in gills than in digestive glands compared to controls Digestive gland showed a significant higher number of carbonylated proteins than gills after NP exposure Protein ubiquitination and carbonylation are therefore independent processes Results showed that NP exposure generated ROS in gill and digestive gland of R decussatus that significantly altered the proteome Results also highlighted the advantage of using redox proteomics in the assessment of protein ubiquitination and carbonylation which may be markers of oxidative stress in R decussatus (C) 2010 Elsevier Ltd All rights reserved
0045-6535
10.1016/j.chemosphere.2010.09.038
Grant Details
Publication Themes