Peer-Reviewed Journal Details
Mandatory Fields
Healy, D,Nash, M,Gorelov, A,Thompson, K,Dockery, P,Belochapkine, S,Madden, J,Rochev, Y
2017
January
International Journal Of Polymeric Materials And Polymeric Biomaterials
Nanometer-scale physically adsorbed thermoresponsive films for cell culture
Published
WOS: 5 ()
Optional Fields
Cell sheet detachment physical adsorption poly (N-isopropylacrylamide) thermoresponsive polymers POLY N-ISOPROPYLACRYLAMIDE PROTEIN ADSORPTION TITANIUM SURFACES ENDOTHELIAL-CELLS DILUTE-SOLUTIONS FOCAL ADHESIONS SHEET RECOVERY IN-VITRO POLY(N-ISOPROPYLACRYLAMIDE) GROWTH
66
221
234
Physical adsorption was used to produce nanometer thick thermoresponsive films with a view to nonenzymatic cell detachment. Two polymers were investigated, poly-(N-isopropylacrylamide) and poly (N-isopropylacrylamide-co-N-tertbutylacrylamide). Substrates were prepared above and below the polymers' LCST to investigate the effect of polymer conformation on the prepared substrates. Endothelial cells were seeded on the prepared films; cell proliferation was higher on the films produced below the polymers' LCST than on those prepared above and cells detached from the surfaces upon temperature reduction. Physical adsorption of poly-(N-isopropylacrylamide)-based films is a viable approach to produce substrates compliant with cell growth and temperature modulated detachment.[GRAPHICS].
10.1080/00914037.2016.1201765
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