Peer-Reviewed Journal Details
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Stenson, C., McNair, A., Curley, M., Smith, T., Gannon, F.
2002
January
Molecular Genetics And Genomics
A role for atlantic salmon HNF-3 in the regulation of Alantic salmon HNF-1
Published
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Optional Fields
hepatic nuclear factor-1/-3 transcriptional network Salmo salar TRANSCRIPTION FACTORS EXPRESSION PATTERNS HEPATOCYTE DOMAIN LIVER TRANSTHYRETIN ENHANCER PROMOTER SEQUENCE SITE
266
832
837
Hepatocyte nuclear factors -1 (HNF-1) and -3 (HNF-3) are hepatocyte-enriched transcription factors that are central to the establishment and maintenance of the liver phenotype in vertebrates. In the present study we demonstrate that, in the Atlantic salmon, asHNF-3 regulates the expression of the gene for asHNF-1. Multiple putative binding sites for asHNF-3 were identified within the 5' flanking region of the HNF-1 gene using a computer-based algorithm, and these were confirmed to be functional by electrophoretic mobility shift assays. In transient transfection assays it was shown that co-expression of asHNF-3 leads to a decrease in the promoter activity of the 5' flanking region of the asHNF- 1 gene.
DOI 10.1007/s00438-001-0602-0
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