Peer-Reviewed Journal Details
Mandatory Fields
Connolly, PF,Fearnhead, HO
2016
October
Febs Journal
DNA-PK activity is associated with caspase-dependent myogenic differentiation
Published
Altmetric: 2WOS: 2 ()
Optional Fields
apoptosis caspase-3 differentiation DNA-damage DNA-PK muscle myogenesis KINASE CATALYTIC SUBUNIT PROTEIN-KINASE MUSCLE DIFFERENTIATION NONSENSE MUTATION DAMAGE GENE H2AX IDENTIFICATION REPLICATION REPAIR
283
3626
3636
Differentiation of myoblasts into myotubes is essential for skeletal muscle development and regeneration. Caspase-3 and caspase-9 are required for efficient myoblast differentiation. The caspase-activated endonuclease activity, CAD, and the DNA-damage repair protein XRCC1 have also been shown to be required to complete differentiation. DNA-damage associated with differentiation is accompanied by phosphorylation of Histone 2AX, an event normally catalysed by kinases ATR, ATM or DNA-PK. However, the kinase responsible for phosphorylation during differentiation is not known. Here we show that inhibition of DNA-PK, but not of ATR or ATM, blocked histone phosphorylation during differentiation. We also show that DNA-PK inhibition and siRNA-mediated DNA-PK knockdown blocked cell fusion. These data implicate a new role for DNA-PK in myogenic differentiation.
10.1111/febs.13832
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