Peer-Reviewed Journal Details
Mandatory Fields
Connolly, JM,Kane, MT,Quinlan, LR,Dockery, P,Hynes, AC
2016
October
Reproduction Fertility And Development
Hypoxia limits mouse follicle growth in vitro
Published
Optional Fields
culture system development growth oxygen preantral VEGF ENCAPSULATED 3-DIMENSIONAL CULTURE PRIMARY OVARIAN-FOLLICLES PRIMORDIAL FOLLICLES STIMULATING-HORMONE PREANTRAL FOLLICLES OOCYTE MATURATION ASCORBIC-ACID DEVELOPMENTAL COMPETENCE SUPPORTS DEVELOPMENT LUTEINIZING-HORMONE
28
1570
1579
Ovarian follicle culture is useful for elucidation of factors involved in the regulation of follicular function. We examined the effects of gas phase oxygen concentration, an oil overlay, serum type and medium supplementation with FSH, insulin-transferrin-selenium (ITS) and l-ascorbic acid on cultured preantral mouse follicle growth in a spherical, non-attached follicle culture system. Follicle growth in 5% oxygen was significantly (P<0.01) inferior to growth in 20% oxygen in terms of follicle diameter. This was likely due to hypoxia, as evidenced by significantly (P<0.05) increased follicle secretion of vascular endothelial growth factor (VEGF), a marker of cell hypoxia. Follicular growth was not (P>0.05) affected by an oil overlay, ITS supplementation or serum type. Culture in medium with 5% mouse serum, 1IUmL(-1) FSH, 25gmL(-1) l-ascorbic acid and 20% oxygen without an oil overlay supported the growth of follicles to a maximum diameter of 380m in 6 days. Compared with mature preovulatory mouse follicles in vivo that often have diameters >500m within the same time frame, in vitro-grown follicles clearly exhibit limited growth. Thus, adequate oxygenation is an essential factor in the process of optimising follicle growth.
10.1071/RD14471
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