Peer-Reviewed Journal Details
Mandatory Fields
Yao, L., Wang, S., Cui, W., Sherlock, R., O'Connell, C., Damodaran, G., Gorman, A., Windebank, A. and Pandit, A.
2009
February
Acta Biomaterialia
Effect of Functionalised Micropatterned PLGA Scaffold on Guided Neurite Growth
Published
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Optional Fields
Guided regeneration Micropatterning Laminin peptide PLGA PERIPHERAL-NERVE REGENERATION SCHWANN-CELL GROWTH RAT SPINAL-CORD INTEGRIN ALPHA-3-BETA-1 PHEOCHROMOCYTOMA CELLS BIODEGRADABLE POLYMERS TOPOGRAPHICAL CONTROL BASEMENT-MEMBRANE PC12 CELLS OUTGROWTH
5
2
580
588
When coaptation is not possible in the repair of nerve injuries, a bridge of biomaterial scaffold provides a structural support for neuronal cell growth and guides nerve regeneration. Poly(lactide-co-glycolide) (PLGA) scaffolds have been widely investigated for neural tissue engineering applications. In order to investigate guided neurite growth, we have fabricated micropatterns on PLGA films using laser ablation methods. The micropatterned PLGA films were coated with collagen type I or laminin peptide (PPFLMLLKGSTR) to promote axon growth. Micropatterned PLGA films provide a guidance effect on both early stage neurite outgrowth and elongation. Small (5 mu m) grooves showed more statistically significant parallel neurite growth compared with larger size grooves (10 mu m). Micropatterned PLGA films coated with laminin peptide showed more parallel neurite growth compared with those coated with collagen type I. Primary neurite number and total neurite length per cell decreased oil micropatterned PLGA films compared with the controls. Neurites showed a preference for growth in the microgrooves rather than on the spaces. This study indicates that surface micropatterned structures with conjugated functional molecules can be used to guide neurite growth. (c) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
1742-7061
DOI 10.1016/j.actbio.2008.09.002
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