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Enright, Anne-Marie; Collins, Gavin; O'Flaherty, Vincent
2007
September
Systematic And Applied Microbiology
Temporal microbial population dynamics in solvent-degrading anaerobic granular sludge from low-temperature (15C) wastewater treatment bioreactors
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Optional Fields
fluorescence in situ hybridizations 16S rRNA gene sequencing low-temperature anaerobic digestion Methanosaeta Methanosarcina IN-SITU HYBRIDIZATION TARGETED OLIGONUCLEOTIDE PROBES BIOLOGICAL TREATMENT COMMUNITY STRUCTURE MICROORGANISMS WASTEWATERS DYNAMICS METHANOL MICROAUTORADIOGRAPHY IDENTIFICATION
30
471
482
Anaerobic sludge granules were obtained from laboratory-scale anaerobic bioreactors used to treat pharmaceutical-like (methanol-, acetone- and propanol-contaminated) wastewater under low-temperature conditions (15 degrees C). The microbial diversity and diversity changes of the sludge samples were ascertained by applying 16S rRNA gene cloning and terminal restriction fragment length polymorphism (TRFLP) analyses, respectively, and using sludge samples from the inoculum, throughout and at the conclusion of the bioreactor trial. Data from genetic fingerprinting correlated well with those from physiological activity assays of the reactor biomass. Specifically, for example, TRFLP profiles indicated the dominance of hydrogenotrophic methanogens within the archaeal community, thus supporting the findings of specific methanogenic activity measurements. TRFLP data supported the hypothesis that the deviation between the replicated reactors, in terms of treatment efficiency, was associated with succession within the microbial communities present, and indicated that community development was linked to both operating temperature and wastewater composition. Fluorescence in situ hybridization (FISH) was also applied, to quantitatively assess the abundance of selected microbial groups, and revealed the underestimation of the abundance Methanosarcina by gene cloning analysis and demonstrated the spatial arrangement of these organisms within the architecture of the lowtemperature solvent-degrading anaerobic biofilms. (c) 2007 Elsevier GmbH. All rights reserved.
DOI 10.1016/j.syapm.2007.03.005
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