Peer-Reviewed Journal Details
Mandatory Fields
Clancy, E,Coughlan, H,Higgins, O,Boo, TW,Cormican, M,Barrett, L,Smith, TJ,Reddington, K,Barry, T
2016
August
Journal Of Microbiological Methods
Development of internally controlled duplex real-time NASBA diagnostics assays for the detection of microorganisms associated with bacterial meningitis
Published
Optional Fields
Haemophilus influenzae Neisseria meningitidis Streptococcus pneumoniae Real-time NASBA IAC Bacterial meningitis SEQUENCE-BASED AMPLIFICATION BLOOD-STREAM INFECTIONS RECOMBINASE POLYMERASE AMPLIFICATION ISOTHERMAL RNA AMPLIFICATION MOLECULAR BEACON PROBES STREPTOCOCCUS-PNEUMONIAE MENINGOCOCCAL DISEASE HAEMOPHILUS-INFLUENZAE LISTERIA-MONOCYTOGENES HIV-1 RNA
127
197
202
Three duplex molecular beacon based real-time Nucleic Acid Sequence Based Amplification (NASBA) assays have been designed and experimentally validated targeting RNA transcripts for the detection and identification of Haemophilus influenzae, Neisseria meningitidis and Streptococcus pneumoniae respectively. Each real-time NASBA diagnostics assay includes an endogenous non-competitive Internal Amplification Control (IAC) to amplify the splice variant 1 mRNA of the Homo sapiens TBP gene from human total RNA. All three duplex real-time NASBA diagnostics assays were determined to be 100% specific for the target species tested for. Also the Limits of Detection (LODs) for the H. influenzae, N. meningitidis and S. pneumoniae duplex real-time NASBA assays were 55.36, 0.99, and 57.24 Cell Equivalents (CE) respectively. These robust duplex real-time NASBA diagnostics assays have the potential to be used in a clinical setting for the rapid (<60 min) specific detection and identification of the most prominent microorganisms associated with bacterial meningitis in humans. (C) 2016 Elsevier B.V. All rights reserved.
10.1016/j.mimet.2016.06.017
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