Other Publication Details
Mandatory Fields
ANET/COS
Molloy AP, Martin FT, Dwyer RM, Griffin TP, Murphy M, Barry FP, O'Brien T, Kerin MJ.
2009
September
Orthopedics
Author
Published
0
Optional Fields
REGENERATE INFARCTED MYOCARDIUM LEFT-VENTRICULAR FUNCTION ISCHEMIC-HEART FAILURE ENDOTHELIAL-CELLS MONONUCLEAR-CELLS STROMAL CELLS RAT MODEL PARACRINE MECHANISMS COLLATERAL PERFUSION CARDIAC-FUNCTION
Bone marrow-derived mesenchymal stem cells (MSCs) have received much attention as a potential treatment for myocardial infarction because of their potential to integrate into the host myocardium and repair the injured heart. The mode of action of stem cell-mediated cardiac repair is still somewhat unclear, including the potential role of MSCs in neovascularization. The objective of this study was to determine the in vitro effect of MSCs on angiogenesis-related endothelial cell (EC) behavior, including migration, monolayer permeability, and vessel formation and stabilization. In a noncontact coculture system, we found that MSCs increase EC proliferation and migration, promoting early events of angiogenesis, while also decreasing EC monolayer permeability. Further, in a time-and dose-dependent manner, MSCs in direct coculture with ECs on Matrigel (TM) can increase the persistence of preexisting vessels by greater than threefold, with complex vessels remaining stable for more than 10 days. The results demonstrate that MSCs play an active role in the cellular processes involved in the formation, stabilization, and maturation of newly formed vessels. Further, these outcomes are not governed solely by either paracrine or direct contact effects and are both time and dose dependent.
2459
2470
DOI 10.1089/ten.tea.2008.0341
Grant Details
Publication Themes