Conference Publication Details
Mandatory Fields
Alexandrov, SA,McGrath, J,Subhash, H,Boccafoschi, F,Giannini, C,Leahy, M,Brown, TG,Cogswell, CJ,Wilson, T
Novel contrast mechanism for label free super-resolution imaging
THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING XXIII
2016
January
Published
1
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Optional Fields
Optical microscopy label-free imaging super-resolution HOLOGRAPHIC MICROSCOPY HIGH-RESOLUTION WIDE-FIELD
We present a novel super-resolution spectral encoding of spatial frequency (srSESF) approach to break the diffraction limit and dramatically improve resolution in lateral direction. The idea is to utilize additional information about the internal structure of the object to resolve features in the lateral direction. The novel contrast mechanism is realized by reconstruction and comparison of the axial spatial frequency (period) profiles at each image point to form superresolution image. As a result, small features, unresolved by conventional microscopy, can be visualized. Numerical simulation and experiments confirm the super-resolution abilities of the srSESF approach without applying any labels.
10.1117/12.2207458
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