Peer-Reviewed Journal Details
Mandatory Fields
Sanami, M,Sweeney, I,Shtein, Z,Meirovich, S,Sorushanova, A,Mullen, AM,Miraftab, M,Shoseyov, O,O'Dowd, C,Pandit, A,Zeugolis, DI
2016
July
Journal Of Biomedical Materials Research Part B-Applied Biomaterials
The influence of poly(ethylene glycol) ether tetrasuccinimidyl glutarate on the structural, physical, and biological properties of collagen fibers
Published
()
Optional Fields
collagen fibers collagen crosslinking starPEG mechanical properties cytocompatibility CHEMICAL CROSS-LINKING IN-VITRO MECHANICAL-PROPERTIES TISSUE REGENERATION FRACTURE-BEHAVIOR BOVINE PERICARDIA LINKED COLLAGEN MATRICES TENDON BIOCOMPATIBILITY
104
914
922
Various chemical, natural, or synthetic in origin, crosslinking methods have been proposed over the years to stabilise collagen fibers. However, an optimal method has yet to be identified. Herein, we ventured to assess the potential of 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate, as opposed to glutaraldehyde (GTA), genipin and carbodiimide, on the structural, physical and biological properties of collagen fibers. The 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate induced an intermedium surface smoothness, denaturation temperature and swelling. The 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate fibers had significantly higher stress at break values than the carbodiimide fibers, but significantly lower than the GTA and genipin fibers. With respect to strain at break, no significant difference was observed among the crosslinking treatments. The 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate fibers exhibited significantly higher cell metabolic activity and DNA concentration that all other crosslinking treatments, promoted consistently cellular elongation along the longitudinal fiber axis and by day 7 they were completely covered by cells. Collectively, this work clearly demonstrates the potential of 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate as collagen crosslinker. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 914-922, 2016.
10.1002/jbm.b.33445
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