Peer-Reviewed Journal Details
Mandatory Fields
Chen, XY,Daniell, TJ,Neilson, R,O'Flaherty, V,Griffiths, BS
2010
September
European Journal Of Soil Biology
A comparison of molecular methods for monitoring soil nematodes and their use as biological indicators
Published
()
Optional Fields
Biodiversity Soil fauna Nematodes Morphological identification Molecular methods REAL-TIME PCR MICROBIAL COMMUNITY STRUCTURE GEL-ELECTROPHORESIS DGGE POLYMORPHISM T-RFLP FEEDING NEMATODES DIVERSITY BIODIVERSITY IDENTIFICATION FRAMEWORK DIFFERENTIATION
46
319
324
Soil fauna, especially soil nematode communities may be used as indicators for monitoring soil biodiversity and ecological processes. A major drawback facing ecologists is the specialised taxonomic knowledge and labour intensive nature of the work required for traditional morphological identification of soil fauna. We review rapid molecular methods, including: DNA Barcoding or sequencing, PCR-DGGE, PCR-TRFLP and real-time PCR, which could enable an empirical assessment of soil nematode assemblages, in relation to their use as monitoring tools. Based on advantages of: high-throughput; ease of comparison between samples; and rapid data analysis, we argue that PCR-TRFLP is well suited to monitoring purposes. (C) 2010 Elsevier Masson SAS. All rights reserved.
DOI 10.1016/j.ejsobi.2010.05.002
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