Peer-Reviewed Journal Details
Mandatory Fields
Nandakumar, V,Bishop, D,Alonas, E,LaBelle, J,Joshi, L,Alford, TL
Ieee Sensors Journal
A Low-Cost Electrochemical Biosensor for Rapid Bacterial Detection
Optional Fields
Biomedical transducers design automation electrochemical processes impedance measurement ESCHERICHIA-COLI O157-H7 IMPEDANCE SPECTROSCOPY LABEL-FREE SALMONELLA IMMUNOSENSOR TYPHIMURIUM
A three-electrode electrochemical biosensor has been developed using printed circuit boards for detecting bacterial contamination. This low-cost, small-size device consists of thin-film gold electrodes and is fabricated using photo-lithography paired with electro-deposition. Pathogen presence is deduced by detection of change in electrical impedance caused by binding of the pathogen to bio-receptors coated on the sensor surface. The biosensor has a total area of similar to 3.2 cm(2) and requires only 100 mu L of test sample for detection. The sensor geometry has been optimized using techniques from Design of Experiments, and the device can be operated using a small ac excitation potential of magnitude 5 mV. The sensor is tested on the common food-borne pathogen Salmonella typhimurium and is able detect bacterial concentrations of the order of 500 CFU/mL within 6 min. In this paper, the design and fabrication of the biosensor is detailed along with the experiments that validate its performance.
DOI 10.1109/JSEN.2010.2055847
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