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O'Leary, AM,Whyte, P,Madden, RH,Cormican, M,Moore, JE,Mc Namara, E,Mc Gill, K,Kelly, L,Cowley, D,Moran, L,Scates, P,Collins, JD,Carroll, CV
2011
May
Food Microbiology
Pulsed Field Gel Electrophoresis typing of human and retail foodstuff Campylobacters: An Irish perspective
Published
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Campylobacter jejuni Campylobacter coli Genotyping PFGE Human isolates Foodstuff isolates Profile heterogeneity SMAI-DEFINED GENOTYPES ANTIMICROBIAL RESISTANCE GENETIC-CHARACTERIZATION ANTIBIOTIC-RESISTANCE NORTHERN-IRELAND BROILER FLOCK JEJUNI POULTRY COLI SPP.
28
426
433
Campylobacter enteritis is a zoonosis, an infectious disease transmissible under normal conditions from vertebrate animals to man, presenting a major global public health burden. In this study, Pulsed Field Gel Electrophoresis (PFGE) was employed to identify common genotypes in a collection of 600 Campylobacter isolates in order to investigate if profiles obtained from retail samples of foodstuffs matched genotypes causing illness in the community in Ireland. The Campylobacters were isolated from retail foodstuffs, and cases of gastroenteritis, over the same 20-month period in three population centres in Ireland. The major observation made was of a high level of PFGE-genotype heterogeneity; 236 Smal discrete genotypes were found in 507 strains successfully analysed. Analysis of the PFGE profiles revealed 22 common profiles amongst food isolates and those causing enteritis in humans. These cojoint PFGE genotypes indicate that 56 (38%) of the human clinical isolates are genetically related to 129 (36%) of the food isolates. The identification of these recurrent PFGE types, in the sampled Campylobacter coli and Campylobacter jejuni populations, indicates that a high proportion of Campylobacter isolates found in foods of animal origin also occur in patients with symptoms of enteritis. This data adds weight to the epidemiological hypothesis that a high proportion of human Campylobacter cases are contracted via the handling and consumption of contaminated foodstuffs, in particular poultry. (C) 2010 Elsevier Ltd. All rights reserved.
DOI 10.1016/j.fm.2010.10.003
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