Peer-Reviewed Journal Details
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Alexander, JC,Pandit, A,Bao, G,Connolly, D,Rochev, Y
2011
January
Lab On A Chip
Monitoring mRNA in Living Cells in a 3D In Vitro Model using TAT-peptide Linked Molecular Beacons
Published
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Optional Fields
MAMMALIAN-CELLS PROBES HYBRIDIZATION EXPRESSION GENE DELIVERY CULTURES OLIGONUCLEOTIDES VISUALIZATION HETERODUPLEX
11
3908
3914
There is a growing need for the development of in vitro 3D cell culture models for assessing newer therapeutics for clinical applications and mechanisms of human pathology. Molecular beacons have been successfully delivered in two-dimensional (2D) systems to monitor, detect, and localize specific mRNA expression in living cells at the single cell level. However, to date the use of molecular beacons in three-dimensional (3D) systems has not been reported. To translate this technology into specific clinical targeted applications, it is critical to develop and demonstrate efficacy in a 3D system. For the first time the use of TAT-peptide conjugated molecular beacons to monitor mRNA in a 3D in vitro system has been reported.
DOI 10.1039/c1lc20447e
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