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Butler, RK,Nilsson-Todd, L,Cleren, C,Lena, I,Garcia, R,Finn, DP
2011
October
Physiology & Behavior
Molecular and electrophysiological changes in the prefrontal cortex-amygdala-dorsal periaqueductal grey pathway during persistent pain state and fear-conditioned analgesia
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Pain Analgesia Context fear conditioning Field potentials Mitogen-activated protein kinase (MAPK) Periaqueductal grey (PAC) Prefrontal cortex (PFC) ANTERIOR CINGULATE CORTEX ACTIVATED PROTEIN-KINASE LONG-TERM POTENTIATION SIGNAL-REGULATED KINASE ELECTRICAL-STIMULATION BASOLATERAL AMYGDALA FORMALIN TEST GRAY RATS PROJECTIONS
104
1075
1081
Fear-conditioned analgesia (FCA) is the reduction in pain responding which is expressed upon re-exposure to a context previously paired with an aversive stimulus. Projections along the prefrontal cortex (PFC)-amygdala-dorsal periaqueductal grey (dPAG) pathway may mediate FCA. However, there is a paucity of studies measuring both molecular and electrophysiological changes in this pathway in rats expressing persistent pain-related behaviour or FCA. Male Lister-hooded rats, with stimulating and recording electrodes implanted in the amygdala and dPAG, respectively, either received or did not receive footshock (0.4 mA) paired with context, followed 23.5 h later by an intraplantar injection of saline or formalin (50 mu L, 2.5%) into the right hindpaw. Thirty minutes post-formalin/saline, rats were re-exposed to the context for 15 min, during which pain-related behaviours were assessed in addition to evoked field potential recordings in the amygdala-dPAG pathway. Immediately after the 15-minute trial, PFC tissue was isolated for measurement of total and phosphorylated extracellular-signal regulated kinase (ERK) by western blotting. Formalin-evoked nociceptive behaviour in non-fear-conditioned rats was associated with increased field potential amplitude in the dPAG and increased relative expression of phospho-ERK in the PFC. These effects were abolished in rats expressing FCA. Fear conditioning in non-formalin treated rats was associated with increased phospho-ERK in the PFC but no change in field potential amplitude in the dPAG. Together, these data suggest differential, state-dependent alterations in electrophysiological activity and ERK phosphorylation along the PFC-amygdala-dPAG pathway during pain, conditioned fear, and FCA. (C) 2011 Elsevier Inc. All rights reserved.
DOI 10.1016/j.physbeh.2011.05.028
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