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Morris, D,Kavanagh, S,Carney, K,MacDomhnaill, B,Cormican, M
Science Of The Total Environment
CapE (capture, amplify, extract): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC)
WOS: 2 ()
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Verocytotoxigenic E. coli Water Filtration Real time PCR 5'-NUCLEASE PCR O157 O26 DIVERSITY STRAINS SPP. O145 O111 O103
Verocytotoxigenic Escherichia coli (VTEC) is associated with a wide spectrum of disease from mild self-limiting diarrhoea to haemolytic uremic syndrome. Contaminated drinking water is accepted as an important route of transmission in Ireland as elsewhere however established methods for detection of VTEC in drinking water have limitations. We describe a sensitive and rapid method for detection of VTEC from large volumes (20 to 30 L) of drinking water based on filtration, enrichment culture of filters and real-time PCR detection of VTEC virulence and O antigen determinants from enrichments. The method has potential applications for other waterborne pathogens. (c) 2016 Elsevier B.V. All rights reserved.
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