Peer-Reviewed Journal Details
Mandatory Fields
Kevin Doherty, Martin Meere, Petri T. Piiroinen
2016
May
Mathematical Biosciences
A Mathematical Model of Aurora B Activity in Prophase and Metaphase
In Press
Optional Fields
Aurora B; Centromere; Mathematical Modeling; Diffusion; Binding
Aurora B kinase is a protein that controls several processes in mitosis when it is found associated with INCENP, Survivin and Borealin in a complex known as the Chromosomal Passenger Complex. Aurora B in complex with INCENP is phosphorylated on three sites, resulting in the full activation of Aurora B. In prophase and metaphase, Aurora B is activated at centromeres, the region of chromatin linking sister chromatids, due to an autophosphorylation mechanism, and it has been hypothesised that Aurora B is activated throughout the cytoplasm due to its concentration at centromeres. In this article, we first develop a time-dependent model of Aurora B activation that does not incorporate spatial variation. This model is used to demonstrate the various qualitative behaviours that the activation of Aurora B is capable of displaying for different model parameters. Next, we develop a spatio-temporal model of Aurora B activation that includes diffusion of soluble Aurora B and binding of Aurora B to immobile centromeric binding sites. This model describes the activation of Aurora B throughout the cytoplasm due to its concentration-dependent activation at centromeres. The models demonstrate the effects that a soluble phosphatase concentration, multisite phosphorylation and diffusion have on the activation of Aurora B.
http://www.sciencedirect.com/science/article/pii/S0025556416300451
10.1016/j.mbs.2016.04.013
Grant Details
College of Science Scholarship
Publication Themes
Informatics, Physical and Computational Sciences