Peer-Reviewed Journal Details
Mandatory Fields
Redondo, JA,Martinez-Campos, E,Plet, L,Perez-Perrino, M,Navarro, R,Corrales, G,Pandit, A,Reinecke, H,Gallardo, A,Lopez-Lacomba, JL,Fernandez-Mayoralas, A,Elvira, C
2016
April
Macromolecular Rapid Communications
Polymeric Gene Carriers Bearing Pendant beta-Cyclodextrin: The Relevance of Glycoside Permethylation on the "In Vitro" Cell Response
Published
WOS: 2 ()
Optional Fields
cationic polymers cyclodextrins gene therapy nonviral gene vectors POLYAMIDOAMINE DENDRIMER ALPHA-CYCLODEXTRIN DELIVERY VECTORS GAMMA-CYCLODEXTRINS CATIONIC POLYMERS CLICK CLUSTERS TRANSFECTION PYRROLIDINE THERAPY CHOLESTEROL
37
575
583
The incorporation of cyclodextrins (CDs) to nonviral cationic polymer vectors is very attractive due to recent studies that report a clear improvement of their cytocompatibility and transfection efficiency. However, a systematic study on the influence of the CD derivatization is still lacking. In this work, the relevance of beta-CD permethylation has been addressed by preparing and evaluating two series of copolymers of the cationic N-ethyl pyrrolidine methacrylamide (EPA) and styrenic units bearing pendant hydroxylated and permethylated beta-CDs (HCDSt and MeCDSt, respectively). For both cell lines, CDs permethylation shows a strong influence on plasmid DNA complexation, "in vitro" cytocompatibility and transfection efficiency of the resulting copolymers over two murine cell lines. While the incorporation of the hydroxylated CD moiety increased the cytotoxicity of the copolymers in comparison with their homopolycationic counterpart, the permethylated copolymers have shown full cytocompatibility as well as superior transfection efficiency than the controls. This behavior has been related to the different chemical nature of both units and tentatively to a different distribution of units along the polymeric chains. Cellular internalization analysis with fluorescent copolymers supports this behavior.
10.1002/marc.201500647
Grant Details
Publication Themes