Peer-Reviewed Journal Details
Mandatory Fields
James McGrath ; Sergey Alexandrov ; Peter Owens ; Hrebesh Subhash ; Martin Leahy
Journal Of Biomedical Optics
In vivo correlation mapping microscopy
Optional Fields
21(4), 046004 (2016),
To facilitate regular assessment of the microcirculation in vivo, noninvasive imaging techniques such as nailfold capillaroscopy are required in clinics. Recently, a correlation mapping technique has been applied to optical coherence tomography (OCT), which extends the capabilities of OCT to microcirculation morphology imaging. This technique, known as correlation mapping optical coherence tomography, has been shown to extract parameters, such as capillary density and vessel diameter, and key clinical markers associated with early changes in microvascular diseases. However, OCT has limited spatial resolution in both the transverse and depth directions. Here, we extend this correlation mapping technique to other microscopy modalities, including confocal microscopy, and take advantage of the higher spatial resolution offered by these modalities. The technique is achieved as a processing step on microscopy images and does not require any modification to the microscope hardware. Results are presented which show that this correlation mapping microscopy technique can extend the capabilities of conventional microscopy to enable mapping of vascular networks in vivo with high spatial resolution in both the transverse and depth directions.
Grant Details
Publication Themes
Biomedical Science and Engineering, Informatics, Physical and Computational Sciences