Peer-Reviewed Journal Details
Mandatory Fields
Sweeney, SM,Bullen, GA,Gillis, RB,Adams, GG,Rowe, AJ,Harding, SE,Tucker, JHR,Peacock, AFA,Murphy, PV
2016
March
Tetrahedron Letters
Coiled coil type neoglycoproteins presenting three lactose residues
Published
WOS: 1 ()
Optional Fields
Scaffold Glycosylated Coiled coil Glycocluster BUILDING-BLOCKS LECTIN-BINDING SUGAR CODE PEPTIDES DESIGN GLYCOPEPTIDES GLYCOCLUSTERS COORDINATION SCAFFOLDS PROTEINS
57
1414
1417
Scaffold design, synthesis and application are relevant for biomedical research. For example, multivalent interactions, such as those between cell surface glycoproteins and lectins can influence the potency and duration of signalling. The spacing between carbohydrates on their native protein scaffold could be important. Herein, the coiled coil design principle is used to generate synthetic coiled coil type glycoproteins, where three lactose residues are grafted to the coil via N-linkages to asparagine. Molecular modelling indicates that the distance between the galactose anomeric carbon atoms on the neoglycoproteins is similar to 30 angstrom. The inclusion of lactose was accommodated in both the final heptad towards the N-terminus, or more centrally in the penultimate heptad. In either case, neither the helicity nor the assembly to the trimeric form was unduly altered by the presence of the disaccharide. (C) 2016 Elsevier Ltd. All rights reserved.
10.1016/j.tetlet.2016.02.005
Grant Details
Publication Themes