Peer-Reviewed Journal Details
Mandatory Fields
Foerster A, Holowacz I, Kumar GBS, Anandakumar S, Wall JG, Wawrzynska M, Paprocka M, Kantor A, Kraskiewicz H, Olsztynska-Janus S, Hinder SJ, Bialy D, Podbielska H, Kopaczynska M
2016
April
Journal Of Biomedical Materials Research Part A
Stainless steel surface functionalization for immobilization of antibody fragments for cardiovascular applications
Published
Optional Fields
aminosilanization surface modification antibody fragments immobilization progenitor cells ENDOTHELIAL GROWTH-FACTOR RAY PHOTOELECTRON-SPECTROSCOPY SINGLE-CHAIN ANTIBODIES IN-STENT RESTENOSIS ORIENTED IMMOBILIZATION FACTOR RECEPTOR-2 ESCHERICHIA-COLI CD34(+) CELLS ACID 3-AMINOPROPYLTRIETHOXYSILANE
104
821
832
Stainless steel 316 L material is commonly used for the production of coronary and peripheral vessel stents. Effective biofunctionalization is a key to improving the performance and safety of the stents after implantation. This paper reports the method for the immobilization of recombinant antibody fragments (scFv) on stainless steel 316 L to facilitate human endothelial progenitor cell (EPC) growth and thus improve cell viability of the implanted stents for cardiovascular applications. The modification of stent surface was conducted in three steps. First the stent surface was coated with titania based coating to increase the density of hydroxyl groups for successful silanization. Then silanization with 3 aminopropyltriethoxysilane (APTS) was performed to provide the surface with amine groups which presence was verified using FTIR, XPS, and fluorescence microscopy. The maximum density of amine groups (4.8*10(-5) mol/cm(2)) on the surface was reached after reaction taking place in ethanol for 1 h at 60 degrees C and 0.04M APTS. On such prepared surface the glycosylated scFv were subsequently successfully immobilized. The influence of oxidation of scFv glycan moieties and the temperature on scFv coating were investigated. The fluorescence and confocal microscopy study indicated that the densest and most uniformly coated surface with scFv was obtained at 37 degrees C after oxidation of glycan chain. The results demonstrate that the scFv cannot be efficiently immobilized without prior aminosilanization of the surface. The effect of the chemical modification on the cell viability of EPC line 55.1 (HucPEC-55.1) was performed indicating that the modifications to the 316 L stainless steel are non-toxic to EPCs. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 821-832, 2016.
10.1002/jbm.a.35616
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