Peer-Reviewed Journal Details
Mandatory Fields
van den Bosch, M,Lowndes, NF
2004
February
Cell Cycle
Remodelling the Rad9 checkpoint complex - Preparing Rad53 for action
Published
()
Optional Fields
DNA damage checkpoint Mec1 Rad9 Rad53 chaperones SSA1/2 DNA-DAMAGE CHECKPOINT BUDDING YEAST RAD9 SACCHAROMYCES-CEREVISIAE CELL-CYCLE CHAPERONE PROTEIN HSP70 PHOSPHORYLATION REPAIR SITES
3
119
122
DNA damage checkpoints are signal transduction pathways that are activated after genotoxic insults to protect genomic integrity. The Rad9 protein functions in the DNA damage checkpoint pathway in Saccharomyces cerevisiae and is essential for the Mec1-dependent activation of the effector kinase Rad53. We recently described the purification of two soluble distinct Rad9 complexes. The large 850 kDa complex consists of hypophosphorylated Rad9 and the chaperone proteins Ssa1/2. This complex is found both in undamaged cells as well as in cells treated with DNA damaging agents. The smaller 560 kDa complex contains hyperphosphorylated Rad9, Ssa1/2 and, in addition, Rad53. This complex forms only in cells with compromised DNA integrity. Once bound to the smaller complex, Rad53 can be activated by in trans autophosphorylation. Here, we propose a model in which the large Rad9 complex is remodelled after a genomic insult by chaperone activity to a smaller Rad53 activating complex.
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