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O'Loughlin, AJ,O'Sullivan, CJ,Ravikumar, N,Friel, AM,Elliott, JT,Morrison, JJ
2005
February
Reproductive Biology And Endocrinology
Effects of thrombin, PAR-1 activating peptide and a PAR-1 antagonist on umbilical artery resistance in vitro
Published
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Optional Fields
ENDOTHELIUM-DEPENDENT RELAXATION RECEPTORS INFLAMMATION CELLS
3
1
7
Background: The non-thrombotic effects of thrombin in cardiovascular tissues, as mediated via the protease activated receptors (PARs), and particularly PAR-1, have been the focus of much recent research. The aims of this study were to evaluate the effects of thrombin, a specific PAR-1 activating peptide (PAR1-AP), and a PAR-1 antagonist on human umbilical artery tone in vitro.Methods: Human umbilical artery samples were obtained from 17 women at term. Arterial rings were suspended under physiologic conditions for isometric recording. The in vitro effects of thrombin (0.5 units/mL to 3 units/mL), PAR1-AP TFLLR-NH2 [10(-9) to 10(-6) M], and PAR-1 antagonist (N-trans cinnamoyl-p-fluoroPhe- p-guanidinoPhe-Leu-Arg-Orn-NH2) [10(-9) M to 10(5) M] on umbilical artery tone were measured.Results: Both thrombin and TFLLR-NH2 exerted a potent cumulative vasodilatory effect on human umbilical artery resistance (P 0.05).Conclusion: These findings highlight a potential role for thrombin and PAR-1 receptors in vascular regulation of feto-placental blood flow in normal pregnancy, and in association with the vascular lesions associated with IUGR and pre-eclampsia.
ARTN 8
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