Peer-Reviewed Journal Details
Mandatory Fields
Hortiguuela, MJ,Aumailley, L,Srivastava, A,Cunningham, C,Anandakumar, S,Robin, S,Pandit, A,Hu, XJ,Wall, JG
2015
December
Polymers For Advanced Technologies
Engineering recombinant antibodies for polymer biofunctionalization
Published
Altmetric: 1WOS: 3 ()
Optional Fields
recombinant protein antibody fragment immobilization biofunctionalization ESCHERICHIA-COLI DOMOIC ACID AFFINITY MATURATION MONOCLONAL-ANTIBODY FRAGMENT BINDING IMMOBILIZATION PROTEINS OPTIMIZATION EXPRESSION
26
1394
1401
The attachment of recognition elements such as antibody fragments to polymeric substrates can be used to mediate cell- or protein-specific interactions. In this work, single-chain Fv (scFv) antibody fragments were isolated against two cell types of interest and expressed in an Escherichia coli expression platform. The scFvs were engineered at their C-terminus to incorporate a cysteine-containing linker, for reaction with maleimide-linked polymers, or a heptasaccharide glycan for complexation with surface amine moieties. Antigen binding of the modified scFvs was unchanged, and expression yields of the glyco-engineered scFvs were similar to the unmodified molecules, while cys-tagged scFv yields varied between scFv variants. Targeted immobilization of the scFvs via either modification resulted in three-to five-fold higher binding of ligands over adsorbed molecules. The study demonstrates a simple and efficient antibody engineering and modification approach for effective targeted immobilization on polymeric substrates. Copyright (C) 2015 John Wiley & Sons, Ltd.
10.1002/pat.3619
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