Peer-Reviewed Journal Details
Mandatory Fields
Houlihan, DD,Dennedy, MC,Morrison, JJ
2010
April
Reproduction
Effects of abnormal cannabidiol on oxytocin-induced myometrial contractility
Published
()
Optional Fields
CANNABINOID RECEPTOR IN-VITRO PREGNANT MYOMETRIUM CB1 RECEPTORS RELAXATION ANANDAMIDE AGONISTS LIGANDS MUSCLE NERVES
139
783
788
The objective of this study was to investigate the effects of abnormal cannabidiol (abn-cbd) on oxytocin-induced myometrial contractility occurring during pregnancy. Isometric tension recordings were performed in isolated myometrial strips from biopsies obtained at elective cesarean section. The effects of cumulative doses of abn-cbd (10(-9) - 10(-5) M) on oxytocin-induced myometrial contractions alone, and on those following pre-incubation with SR 144528, AM 251, methylene blue, and iberiotoxin were measured, and dose response curves were constructed. The pD(2) (-log EC50) values and the maximal inhibitory (MMI) values that were achieved were compared for each tissue type. Abn-cbd exerted a potent relaxant effect on oxytocin-induced myometrial contractions in vitro. Pre-incubation with the guanylate cyclase inhibitor, methylene blue, and the BKCa channel antagonist, iberiotoxin, significantly attenuated this effect (for pD(2), P<0.01; for MMI, P<0.01). Abn-cbd exerts a potent inhibitory effect on human uterine contractility. This effect is partially mediated through modulation of guanylate cyclase and activation of BKCa channel activity. These findings have implications for physiologic regulation of myometrial quiescence. Reproduction (2010) 139 783-788
DOI 10.1530/REP-09-0496
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