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O'Byrne, CP,Feehily, C,Ham, R,Karatzas, KAG
2011
January
Journal Of Microbiological Methods
A modified rapid enzymatic microtiter plate assay for the quantification of intracellular gamma-aminobutyric acid and succinate semialdehyde in bacterial cells
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Listeria monocytogenes GABA Succinate semialdehyde Acid tolerance GABase ESCHERICHIA-COLI LISTERIA-MONOCYTOGENES IN-VITRO GABA SYSTEM INHIBITION RESISTANCE
84
137
139
The GABase assay is widely used to rapidly and accurately quantify levels of extracellular gamma-aminobutyric acid (GABA). Here we demonstrate a modification of this assay that enables quantification of intracellular GABA in bacterial cells. Cells are lysed by boiling and ethanolamine-O-sulphate, a GABA transaminase inhibitor is used to distinguish between GABA and succinate semialdehyde. (C) 2010 Elsevier B.V. All rights reserved.
DOI 10.1016/j.mimet.2010.10.017
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