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Nash, ME,Carroll, WM,Nikoloskya, N,Yang, RB,Connell, CO,Gorelov, AV,Dockery, P,Liptrot, C,Lyng, FM,Garcia, A,Rochev, YA
2011
June
Acs Applied Materials & Interfaces
Straightforward, One-Step Fabrication of Ultrathin Thermoresponsive Films from Commercially Available pNIPAm for Cell Culture and Recovery
Published
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poly-N-isopropylacrylamide cell recovery spin coating surface properties MECHANICAL-PROPERTIES POLYSTYRENE SURFACES POLYMER SURFACES ADHESION COPOLYMERS DETACHMENT TISSUES
3
1980
1990
The use of thermoresponsive surfaces as platforms for cell culture and cell regeneration has been explored over the last couple of decades. Poly-N-isopropylacrylamide (pNIPAm) is a well characterized thermoresponsive polymer which has an aqueous lower critical solution temperature (LCST) in a physiologically useful range, which allows it to reversibly attract (T 32 degrees C). It is this phenomenon that is exploited in temperature-controlled cell harvesting. pNIPAm coatings are generally poorly cell compatible and a number of complex or expensive techniques have been developed in order-to overcome this issue. This study seeks to design a simple one-step system whereby commercially sourced pNIPAm is used to achieve similar results. Films were deposited using the operationally simple but rheologically complex spin coating technique. Reversible temperature modulated cell adhesion was achieved using a variety of different cell lines. This system offers a simplistic and cheaper alternative to methods used elsewhere.
DOI 10.1021/am200204j
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