Peer-Reviewed Journal Details
Mandatory Fields
Eglon, M,McGrath, B,O'Brien, T,Armstrong, D
2010
August
Methods Mol Biol
HPLC Purification of Adenoviral Vectors
Published
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Optional Fields
Adenovirus Gene therapy vectors Chromatography Anion exchange Gel filtration FLOW-CYTOMETRY TYPE-5 ASSAY CHROMATOGRAPHY TITRATION PARTICLES
594
395
408
Adenoviruses are attractive vectors for gene therapy where short-term transgene expression is required. In order to nicer the clinical requirements of adenovirus for use beyond the laboratory, advanced methods are required for the purification and quantitation of recombinant adenoviral vectors (rAd). Chromatographic systems offer the advantages of linear scalability and reproducibility, and this method describes a laboratory-scale process based on liquid chromatography, which can be technically transferred and readily scaled-up according to the demands of the laboratory or clinic in which it will be used.
10.1007/978-1-60761-411-1_28
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