Peer-Reviewed Journal Details
Mandatory Fields
Halvey, PJ,Watson, WH,Hansen, JM,Go, YM,Samali, A,Jones, DP
2005
March
Biochemical Journal
Compartmental oxidation of thiol-disulphide redox couples during epidermal growth factor signalling
Published
()
Optional Fields
epidermal growth factor (EGF) glutathione redox signalling thioredoxin-1 thioredoxin-2 HUMAN MITOCHONDRIAL THIOREDOXIN HUMAN CELL-LINES IMMUNOHISTOCHEMICAL LOCALIZATION HYDROGEN-PEROXIDE DNA-BINDING STRESS GLUTATHIONE ACTIVATION ENDOCYTOSIS EXPRESSION
386
215
219
Exogenously added ROS (reactive oxygen species) cause generalized oxidation of cellular components, whereas endogenously generated ROS induced by physiological stimuli activate discrete signal transduction pathways. Compartmentation is an important aspect of such pathways, but little is known about its role in redox signalling. We measured the redox states of cytosolic and nuclear Trx1 (thioredoxin-1) and mitochondrial Trx2 (thioredoxin-2) using redox Western blot methodologies during endogenous ROS production induced by EGF (epidermal growth factor) signalling. The glutathione redox state was measured by HPLC. Results showed that only cytosolic Trx1 undergoes significant oxidation. Thus EGF signalling involves subcellular compartmental oxidation of Trx1 in the absence of a generalized cellular oxidation.
10.1042/BJ20041829
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