Peer-Reviewed Journal Details
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Srivastava, A,Cunningham, C,Pandit, A,Wall, JG
2015
May
Macromolecular Bioscience
Improved Gene Transfection Efficacy and Cytocompatibility of Multifunctional Polyamidoamine-Cross-Linked Hyaluronan Particles
Published
WOS: 9 ()
Optional Fields
conjugated polymers drug delivery systems hyaluronic acid intervertebral disk poly(amidoamine) ANTIBODY FRAGMENT PAMAM DENDRIMERS CELLULAR UPTAKE DOMOIC ACID DELIVERY SIRNA BINDING CYTOTOXICITY DEGENERATION THERAPY
15
682
690
We describe a multi-functional, cationic hyaluronic acid (HA)-based gene carrier with improved transfection over non-cross-linked HA, and negligible cytotoxicity. Cationized particles are developed by cross-linking HA chain carboxyl groups with polyamidoamine amine termini to produce well segregated particles of 350-400nm with a surface charge density of +2mV, compared with -35mV for non-cationized particles. A tethered antibody fragment retains ligand binding for cell targeting. Cationized and antibody-linked particles complex plasmid DNA efficiently and the cationized particles successfully deliver reporter genes to bovine intervertebral disk cells as an intervertebral disk regeneration model.
10.1002/mabi.201400401
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