Peer-Reviewed Journal Details
Mandatory Fields
Chandran, S,Cairns, MT,O'Brien, M,Smith, TJ
2014
August
Molecular And Cellular Endocrinology
Transcriptomic effects of estradiol treatment on cultured human uterine smooth muscle cells
Published
Optional Fields
17 beta-Estradiol Myometrium Smooth muscle contraction Estrous Pregnancy Global gene expression HUMAN MYOMETRIUM GENE-EXPRESSION RAT MYOMETRIUM CDNA MICROARRAY LATE PREGNANCY MOUSE UTERUS ESTROGEN RECEPTOR CONTRACTILITY PARTURITION
393
16
23
Contractility of the myometrial smooth muscle cells during the estrous cycle and pregnancy is modulated by estrogen but the temporal expression of estrogen (relative to progesterone) and the type of contraction involved are distinctly different in pregnancy and estrous. This in vitro cell culture study investigated the global gene expression profile of human uterine smooth muscle cells (hUtSMCs) following 17 beta-estradiol (E2) treatment. In response to E2 treatment 540 genes, many of which have not been previously described as estrogen responsive, were identified as significantly differentially expressed. These genes are involved in biological processes that include muscle contraction, cell migration and adhesion, apoptosis and phosphorylation. Evidence from this study suggests that 17 beta-estradiol may have effects that are contrary to an overall contraction phenotype. The hUtSMC in vitro culture system is a useful model to investigate steroid effects on smooth muscle cells and may provide additional clues as to how smooth muscle cells behave in vivo. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
10.1016/j.mce.2014.05.020
Grant Details
Publication Themes