Peer-Reviewed Journal Details
Mandatory Fields
Maher, M,Glennon, M,Martinazzo, G,Turchetti, E,Marcolini, S,Smith, T,Dawson, MT
1996
September
Journal Of Clinical Microbiology
Evaluation of a novel PCR-based diagnostic assay for detection of Mycobacterium tuberculosis in sputum samples
Published
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Optional Fields
POLYMERASE CHAIN-REACTION CLINICAL-SAMPLES DNA AMPLIFICATION IDENTIFICATION SPECIMENS SEDIMENTS
34
2307
2308
We report on a PCR-based assay we have developed for the detection of Mycobacterium tuberculosis in sputum samples. One hundred sputum specimens, which included 34 culture-positive and 66 culture-negative specimens, were evaluated with this system. Of the 34 culture-positive specimens, 31 were PCR positive, and 60 of the culture-negative specimens were PCR negative. An internal standard has been included in the assay system to monitor PCR inhibition and to confirm the reliability of the PCR assay.
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