Peer-Reviewed Journal Details
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Rowe SE, O'Gara JP
2016
Unknown
Methods in molecular biology (Clifton, N.J.)
Electrophoretic Mobility Shift Assays.
Published
()
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1373
155
167
Experimental demonstration of regulatory protein interactions with the sequences upstream of potential target genes is an important element in gene expression studies. These experiments termed electrophoretic mobility shift assays (EMSAs) provide valuable insight into the mechanism of action of transcription factors. EMSAs combined with downstream applications such as transcriptional analysis help uncover precisely how regulatory proteins control target gene expression. This chapter comprises a guideline for expression and purification of recombinant transcription factor proteins followed by a detailed protocol for EMSAs..
10.1007/7651_2015_277
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