Conference Contribution Details
Mandatory Fields
Ryder, A.G.
PEGS Europe: Protein & Antibody Engineering Summit
Comprehensive, Quantitative Bioprocess Productivity Monitoring Using Fluorescence EEM Spectroscopy and Chemometrics.
Lisbon Portugal
Invited Oral Presentation
Optional Fields
02-NOV-15
06-NOV-15
Multi-dimensional fluorescence spectroscopy (MDFS) was used for quantitative predictive analysis of glycoprotein production and content in CHO cell fed-batch process. MDFS spectra of complex solutions were sensitive to compositional change and as cultivation progressed, amino acid, and glycoprotein product emission varied as the chemical composition changed and culture progress could be monitored quantitatively using a variety of multivariate analysis techniques. In one case, unique dityrosine emission from product glycoprotein could be used to follow product formation. The MDFS variance could also be used to generate quantitative predictive models of process performance based on glycoprotein yield.
Science Foundation Ireland: IvP
Publication Themes